Glutamine prevents the decrease of intestinal calcium absorption caused by oxidative stress preserving molecular structures of both transcelular and paracelular pathways

PEREZ, ADRIANA; DIAZ DE BARBOZA GABRIELA EDITH; MOINE LUCIANA BEATRIZ; TOLOSA DE TALAMONI, NORI GRACIELA

Mar del Plata

Congreso; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica 2018; 2018

Sociedad Argentina de Investigación Clínica

Calcium (Ca2+) is absorbed in intestine through transcellular and paracellular pathways. We have previously shown that Menadione (MEN) causes oxidative stress and apotosis in enterocytes, decreasing intestinal Ca2+ absorption. Glutamine (GLN), the main nutrient of these cells, has antioxidant and antiapoptotic properties. Our aim was to study the ability of GLN to maintain the functionality of intestinal Ca2+ absorption pathways decreased by MEN. In an animal model using Gallus gallus domesticus, the effect of drugs on intestinal Ca2+ absorption was studied by intestinal loop technique ligated in situ, in presence or absence of Ruthenium Red (RR, inhibitor of TRPV5/6, molecule of transcellular pathway), and gene and protein expression of components of both absorption pathways by PCR and western blot. The data were analyzed by one-way ANOVA and Tukey´s test post hoc; differences were considering significant at p