PATERNAL MODERATE ALCOHOL CONSUMPTION MODIFIES MOUSE PERI-IMPLANTATION EMBRYO DEVELOPMENT

LUCILA GOTFRYD; FERNANDO FUENTES; MELISA CELESTE SANCHEZ; GABRIELA SALAMONE; JUAN CARLOS CALVO; ELISA CEBRAL; VANINA FONTANA

Jornada; Jornadas Interdisciplinarias de Química Biológica; 2017

Departamento de Química Biológica, FCEN, UBA

Previously, we showed that paternal alcohol intake partially inhibits spermcapacitation, increases nuclear decondensation rate and deregulates thedynamics of pronucleus formation and fertilization1,2,3. Aim: To evaluate the effectof paternal alcohol consumption and its consequences on mouse early embryodevelopment focusing on trophoblast (TB) differentiation and inner cell mass (ICM)as critical embryo stages invading maternal decidua. Methods: CF-1 fertile maleswere exposed (treated group, T) or not (control group, C) to 15% (v/v) ethanol indrinking water ad libitum for 15 days. They were mated with a fertile nontreatedCF-1 female (1:1). Positive mating females were sacrificed at day 2 of gestation toobtain 2 cell embryos which, then, were cultured for 7 days. Embryo differentiation,growth and morphology were evaluated during pre- and peri-implantation phasesin vitro. Then, embryos were classified as type A (ICM: protruding aggregates ofcompact cells; TB: symmetric monolayer of flat and elongated cells) or type B(ICM: disaggregated, few scattered or no cells, TB: asymmetric trophoblastoutgrowth). Frequency differences between C and T were tested by Fisher´s exacttest. Results: Male alcohol consumption for 15 days delayed the embryodifferentiation and growth by detention/fragmentation and altered the embryomorphology. Differences between type A and B distribution in C and T werestatistically significant for ICM and TB in both groups. For ICM: Type A 53% C vs10% T (p<0.0001), for TB: 60% C vs 26% T (p<0.01). When we evaluated thetrophoblast growth area at day 7 of culture there were no significant differencesbetween both groups. Conclusion:Paternal alcohol consumption impairs earlymouse embryo peri-implantation, affecting ICM and TB development. Theseeffects might be crucial for further embryo survival, considering that ICMparticipates in the embryo formation and TB plays a relevant role in placentaldevelopment.Referencias:[1] Sánchez MC, et al. Reproduction enviado. 2017[2] Sánchez MC, et al. Systems Biology in Reproductive Medicine, 2013, 59(2):82-90.[3] Fontana V, et al. Journal of Molecular Histology, 2012, 43 (5): 487?496.Agradecimientos y apoyofinanciero:This work was supported by UBACYT 2016-2018, PICT 2012-2015