INVESTIGADORES
IRIBARREN Pablo
congresos y reuniones científicas
Título:
REGULATION OF MICROGLIAL CELL CYTOKINE PRODUCTION BY TYPE 2 CYTOKINES
Autor/es:
GAVIGLIO, EA; SORIA, JA; ARROYO, DS; IRIBARREN, P
Lugar:
Buenos Aires
Reunión:
Congreso; First French-Argentine Immunology Congress; 2010
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
Inflammation plays an
important role in central nervous system (CNS) infection and the progression of
neurodegenerative diseases. Microglial cells are phagocytic myeloid cells located in the nervous parenchyma playing
a key role in the initiation, progression and resolution of neuroinflammation.
It is well known that
microglial cells play a key
role in mediating inflammatory processes in the CNS, which are associated with
various neurodegenerative diseases. Given the potential beneficial effects of
anti-inflammatory drugs and cytokines on the treatment and prevention of neurodegenerative
diseases, we investigated the role of IL-4 and IL-13 in the regulation of
cytokine production by microglial cells. First of all,
we evaluated the effects of stimulation of mouse microglial cell lines with IL-4, IL-13 or INF-gamma, on the
production of TNF alpha, IL-10 and IL-27. Both, IL-4 and IL-13 failed to induce
the secretion of TNFalpha or IL-27 (p NS).
However, IL-4 induced the secretion of the anti-inflammatory cytokine IL-10.
In another set of
experiments we tested if IL-4 or IL-13 were capable to inhibit the cytokine
production of microglial cells activated by
pro-inflammatory molecules, such as lipopolysaccharide (LPS) and INF gamma. We found that IL-4 inhibited the
production of IL-6 on LPS-stimulated microglial cells (p<0.01). The effect of IL-4 was blocked by a
monoclonal antibody specific to IL-4 (p<0.01).
These preliminary
results suggest that type 2 cytokines are effective modulators of cytokine
production by activated microglial cells, than inducers
of cytokine production in resting microglial cells. Interestingly, it seems that IL-4 is able to
selectively induce the secretion of IL-10, a molecule important in the
resolution of inflammation.
Additional
experiments are currently performed to evaluate the effects of Type 2 cytokines
on the production of neurotrophic factors.