GABAB receptor deletion in Kiss1 neurons/cells alters body weight and anogenital index

DI GIORGIO NP; TABARES FLORENCIA; BIZZOZZERO MARIANNE; BOURGUIGNON N; BERNHARD BETTLER; LIBERTUN CARLOS; LUX LANTOS V

Toronto

Congreso; . 9th International Congress of Neuroendocrinology; 2018

International Society of Neuroendocrinology

GABAB receptor deletion in Kiss1 neurons/cells alters body weight and anogenital indexNoelia P. Di Giorgio1, Florencia N. Tabares1, Marianne Bizzozzero1, Nadia S. Bourguignon1,Bernhard Bettler2, Carlos Libertun1, 3 and Victoria Lux-Lantos1,*1Institute of Biology and Experimental Medicine-CONICET, Buenos Aires, Argentina.2University of Basel, Switzerland.3Department of Physiology, Faculty of Medicine, University of Buenos Aires, Buenos Aires, Argentina.*: Presenting authorFemale, global GABAB1KO mice show reproductive alterations with disrupted estrous cycles and compromised fertility. GABAB1KO mice co-express GABAB receptors (GABABRs) in Kiss1 neurons and show a dramatic increase in Kiss1 expression in male and female extrahypothalamic areas (amygdala, BNST); its expression did not differ in AVPV-PeN/ARC. To establish the impact of GABABRs on kisspeptin physiology we developed a strain of mice with specific deletion of GABABRs in Kiss1 cells/neurons and characterized them from reproductive and metabolic perspectives.Kiss-Cre mice (Jackson?s Lab) were crossed with GABAB-floxed mice (donated by Dr. Bettler) to obtain Kiss1-GABAB1KO (KO) mice. Body weight (BW) was evaluated from postnatal day (PND) 7 to PND84. Sexual differentiation was evaluated by ano-genital distance (AGD) on PND7, PND14 and PND21 and relativized to BW (AG-index).KO females had increased BW [BW (g) PND84: F-KO=29.5±1.0 (n=6) vs F-WT=25.4±1.2 (n=8), p