14-3-3g AND b HAVE OPPOSITE ROLES IN ADIPOGENIC DIFFERENTIATION

GOJANOVICH, ALDANA D.; MASONE, DIEGO; BUSTOS, DIEGO MARTÍN; UHART, MARINA

Buenos Aires

Congreso; Runion Conjunta de Sociedades de Biociencias; 2017

SAIB

The 14-3-3 protein family regulates several cellular processes including differentiation. Regarding adipogenic differentiation, this family binds to regulatory proteins involved in adipogenesis as TAZ, PPARg2, Lipin-1 and Seipin. 14-3-3 proteins bind their targets through specific phospho-Serine/ Threonine residues. Among the seven highly conserved mammalian family members, specifically 14-3-3g and b mRNA and protein levels increase during adipogenic differentiation of 3T3-L1 preadipocytes. To investigate the role of these paralogs in adipogenesis, we decreased their levels by using lentiviral transduction particles containing short hairpin RNAs (shRNAs) specific to silence the expression of both of these paralogs, and the ZsGreen protein as a marker of transduced cells. 3T3-L1 cells were infected with these vectors, and then induced to the adipogenic linage during 7 days. Adipogenic differentiation was evaluated by Oil Red O staining of lipid droplets followed by confocal microscopy analysis. The results revealed an increment of number and size of lipids droplets after 14-3-3g silencing compared to neighboring cells that were not infected. The opposite effect was observed upon 14-3-3b silencing, where a pronounced decrease in lipid droplets number and sizes was evident in transduced cells. Taken together our findings suggest that 14-3-3g and b differentially regulate 3T3-L1 adipogenesis.