Cell encapsulation in droplets generated by microfluidic devices

PEÑAHERRERA, A.; PEREZ, C.; DE SÀ CARNEIRO, I.; ROSERO, G.; LA GRECA, A.; LOMBARDI, A.; SANTIN VELAZQUE, N. L.; BIANI, M. C.; LUZZANI, C. D.; MIRIUKA, G. S.; PEREZ, M.; LERNER, B.

San Carlos de Bariloche, Argentina

Congreso; Congreso Internacional de Metalurgia y Materiales; 2018

CONAMET

Water-in-oil emulsions are obtained when an aqueous phase is dispersed in an oil carrier phase supplemented with a surfactant to stabilize the emulsion. However, the laminar flow of fluids in microchannels is not sufficient to generate droplets with controlled volume. The production ofmonodisperse emulsions (volume variation less than 5%) requires defined geometries. In this study, the flow focus architecture was applied to design microchannels to produce droplets with a size of 135.55 ± 3.30 μm. The oil phase flow (fluorinated oil HFE7500 + 5% surf) was adjusted to 1.15 ml / h -1 while the aqueous phase flow (human Pluripotent stem cells (hPSC) FN-FUCCI cells in E8 Flex medium) was adjusted to 0.85 m/.h-1. To detect the individual cell encapsulation and the viability of cells, a modification of the cellular DNA was used through the transfection of different plasmids h2b, which produced an autoflurescence in the cells trapped in the droplets depending on the cell cycle.