Platelets bioenergetics as a potential biomarker for Alzheimer's disease

PRESTIA, FEDERICO ARIEL; MARTINO ADAMI, PAMELA VICTORIA; GALEANO, PABLO; CASTAÑO, EDUARDO MIGUEL; CUELLO, AUGUSTO CLAUDIO; MORELLI, LAURA

Ciudad Autónoma de Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedad Argentina de Investigación Clínica (SAIC)

onth-old were anesthetized, beheaded and blood (10 ml) recovered in sterile tubes containing Citrate Buffer/Dextrose (pH 7.4). PLTs were isolated by sequential centrifugation at room temperature to avoid activation. Quantification was performed by hematologic analyzer (Mindray 5800). 15 x 106 PLTs/well were seeded in XFp cell culture microplates and centrifuged (1500g/15 min), after that supernatant was removed and Mito Stress and Glyco Stress tests performed. We set-up a method to evaluate mitochondrial activity in PLTs and is able to plot profiles to compare CNT vs. Tg(+/-). By contrast to synaptosomes, PLTs do not have spare respiratory capacity, it means that are not involved in processes that require extra ATP when energy demand increases, such as synaptic functionality and cognition. This result is in agreement with the physiological role of PLTs. Preliminary data show that bioenergetic parameters of PLTs from 6 month-old Tg(+/-) are similar to CNT. Our results are in the context of a transgenic model and further validation is required in PLTs derived from blood of AD and CNT subjects to determine the relevance of PLTs bioenergetic as a peripheral biomarker for AD.