EFFECT OF RESVERATROL ON THE MITOCHONDRIAL ACTIVITY AND LIPID PEROXIDATION OF VITRIFIED BOVINE EMBRYOS

SERGIO MORADO; STEPHANIA MADRID GAVIRIA; RODRIGO URREGO; ALBEIRO LÓPEZ-HERRERA; GIOVANNI RESTREPO; JULIÁN ECHEVERRI; PABLO CETICA

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Despite being the most commonly used method for embryo cryopreservation, vitrification hasbeen reported to increase reactive oxygen species (ROS) production and todiminish reduced glutathione content leading to deleterious effects on cells.To prevent oxidative damage, several antioxidants have been added tovitrification media. The aim of this study was to determine the effect ofresveratrol on mitochondrial activity and lipid peroxidation of vitrifiedbovine embryos. Cumulus-oocyte complexes were cultured in medium 199 with theaddition of fetal calf serum and gonadotropins at 39°C, 5% CO2 inhumidified air for 22h and then co-incubated with a suspension of 2x106motile spermatozoa/ml for 20h in IVF-mSOF. Then, presumptive zygotes were transferredto IVC-mSOF medium with or without the addition of 0.5µM resveratrol. At day 7,blastocysts were vitrified by the minimum volume method in the presence orabsence of 0.5µM resveratrol. A group of non-vitrified blastocysts was used ascontrol. Mitochondrial activity and mitochondrial O2·- production were determined inthe control and in different groups of vitrified blastocysts by MitotrackerGreen FM/MitoSOX Red dual stain, while lipid peroxidation was determined byBodipy. Blastocysts subjected to vitrification/warming presented highermitochondrial activity and O2·- production than fresh blastocysts (P