DIFFERENTIAL ACTIVITY AND EVALUATION OF PROLIFERATION IN THE OVARY OF A SUDAMERICAN BAT, EUMOPS PATAGONICUS (CHIROPTERA: MOLOSSIDAE): PRELIMINARY RESULTS

RODRIGUEZ, F.E.; OLEA, GB.; AGUIRRE, MV.; LOMBARDO DM.

Ciudad Autónoma de Buenos Aires

Jornada; XX Jornada Anual de la Sociedad de Biología (SAB )2018; 2019

Sociedad Argentina de Biología

The order Chiroptera comprise a great diversity about reproductive patterns; even we could affirm they enclose all the variations in themammalians. In the Molossidae order; studies have been done on the structure of the female reproductive system and ovary dynamic inspecies such as Molossus fortis, Molossus rufus, Molossus molossus, and Mops condylurus. A full dominance of right ovary in this orderis well known, but the explanation about that it has not yet been known. The present study aimed to characterize the ovarian activity ofEumops patagonicus in different seasons and reviewed the proliferative activity in both ovaries. This study was realized using 20specimens obtaining of wildlife, they were anesthetized and euthanized to isolate the reproductive tract. The material was processedfollowing the conventional technique to obtain histological sections and colored whit hematoxylin-eosin. A part of sections was cut in3μm and was processed following the immunohistochemistry technique using the streptavidin-biotin method to evaluate the proliferativeactivity in both ovaries whit PCNA antibody (1:100). The right ovary (RO) and left ovary (LO) were analyzed, in both the cortical zonewith follicles and the medullary region with scarce interstitial glandular tissue (IGT) was distinguished. Regarding the composition of thecortex, along the seasons, only bare oocytes l forming nests, primordia, primary and secondary follicles were found on LO but no oneGraafian follicle. The medullary region was scarce. Nevertheless, on RO it was possible to observe the structures of folliculogenesis aswell as a biovular follicle in winter. Concerning the IGT, it was found in greater quantity than the LO but in a lesser proportion to what isdescribed for M. rufus. In winter recent pregnant females were found, the RO with a corpus luteum occupying a large part of the ovary.These preliminary results agree with the ovarian and functional asymmetry. In the RO, the stages of folliculogenesis were observed,including a corpus luteum on the same side of the pregnant horn. About the proliferative condition, in both ovaries we could see positivenucleus to PCNA. But in RO was higher in number if compared the same type of follicle. We conclude that E. patagonicus presentsmorphological and functional ovarian asymmetry as well as other members of the order.