HYPEROSMOLAR STRESS MODULATES EXTRAVILLOUS TROPHOBLAST CELLS DIFFERENTIATION

MARTINEZ NORA.; REPPETTI JULIETA.; DAMIANO ALICIA E.

Chillan

Congreso; III Meeting on Research and Innovation in Vascular Health and V Meeting on Hypertension in Pregnancy; 2019

GRIVAS

HYPEROSMOLAR STRESS MODULATES EXTRAVILLOUS TROPHOBLAST CELLS DIFFERENTIATIONThe placenta is a transient organ which serves important functions during the development of the fetus. Trophoblast cells develop along a multilineage differentiation pathway, and their growth and differentiation are essential to the establishment and maintenance of pregnancy.The proliferation, migration, invasion and the remodeling of the spiral arteries by the extravillous trophoblast (EVT) are critical processes for a successful pregnancy. Alterations in any of these events can lead to gestational pathologies such as preeclampsia. Many authors have demonstrated that the hyperosmolarity of the medium induced cells differentiation in different tissues.Our hypothesis is that the stress hyperosmolar modulates EVT during placentation.The aim of this study was to explore the effect of the stress hyperosmolar on the EVTs migration, invasion and endovascular differentiation.Swan-71 cell line (human extravillous trophoblast cells) was cultured in complete DMEM-F12 and sucrose hyperosmolar solution was added for 24 h.Cell migration was evaluated by the wound healing assay, activity of metalloproteinases (MMPs) by zymography, the invasion was evaluated in transwells pre-coated with Matrigel® and endovascular differentiation was analyzed by the formation of tube-like structures in plates coated with Matrigel.In hyperosmolar conditions, Swan-71 cell migration, MMPs activity, and invasion decreased (33 ± 4%, 75 ± 8%, and 45 ± 6%, respectively compared to iso-osmolar condition; P