MLC as a Useful Method for Amoxicillin, Ampicillin, Cloxacillin and Dicloxacillin Determination in Pharmaceuticals and Urine.

JAUME ALBIOL CHIVA; JOSEP ESTEVE ROMERO; JUAN PERIS-VICENTE; JESUS JAVIER IBORRA MILLET; DIEGO ENRIQUE KASSHUA; MICAELA FLORES; GERARDO CASTRO OCAMPO; DEVASISH BOSE; SAMUEL CARDA BROCH; EVA MATEO

Cannes

Simposio; 32nd International Symposium on Chromatography ISC; 2018

Penicillin are used to treat a wide range of infections caused by certain susceptible bacteria, including Streptococci, Staphylococci, Clostridium, Neisseria, and Listeria genera. Penicillin antibiotics are derived from Penicillium fungi and can be taken orally or via injection. Penicillins have antibacterial activity via inhibition of bacterial cell wall synthesis. The aim of this study was developed a direct injection liquid chromatographic method for simultaneous determination of Amoxicillin, Cloxacillin and Dicloxacillin in pharmaceutical formulation and urine, using hybrid micellar mobile phases.The four penicillins were analysed using a Zorbax C18 reversed-phase column and detected at 210 nm. These antibiotics were separated by an interpretive optimisation procedure based on the accurate description of the retention and shape of the chromatographic peaks. Antibiotics were eluted in less than 16 min without interfering the urine protein band or endogenous compounds, using asa mobile phase 0.11 M sodium dodecyl sulphate-6% propanol-0.01 M NaH2PO4 buffered at pH 3. The method was validated according to the Food and Drug Administration guideline, including analytical parameters such as linearity (R2>0.993), intra- and inter-day precisions (RSD, %: 0.1-4.4 and 1.2-5.9, respectively), and robustness for the four compounds. This method is sensitive enough for the routine analysis of penicillins at therapeutic urine levels, with limits of detection in the 1.5-15 ng mL -1 range and limits of quantification of 50 ng mL -1 . Recoveries in a micellar medium and a spiked urine matrix were in the 92.4-108.2% and 96-110% ranges, respectively.Finally, the method was successfully applied to determine these penicillin antibiotics in urine samples and pharmaceutical formulations.