POTENTIAL ROLE OF SPEXIN IN MODULATING THE INNATE IMMUNE SYSTEM DURING OBESITY?

GAMBARO SABRINA ELIANA; GIORDANO ALEJANDRA; ZUBIRÍA, MARÍA GUILLERMINA; ANDREA PORTALES; ALZAMENDI ANA; RUMBO, MARTIN; GIOVAMBATTISTA, ANDRÉS

Mar del Plata

Encuentro; Reunión Conjunta de la Sociedad Argentina de Investigación Clínica (SAIC), la Sociedad Argentina de Inmunología (SAI) y la Sociedad Argentina de Fisiología (SAFIS); 2018

Sociedad Argentina de Investigación Clínica

Spexin (SPX) is a new adipokine involved in many processes, suchas energy balance, lipid metabolism, glucose homeostasis andweight control. SPX plasma levels were decreased in obesity/metabolic syndrome (MS) cohorts. Therefore, the hallmarks of obesityand MS are low chronic inflammation and adipocyte hypertrophy.Thus, the aim of this work was to evaluate SPX as able to modulatethe innate immune system in obese mice. Swiss mice were supplemented with a fructose rich diet (FRD, 20%w/v) or tap water for10weeks. Ten days prior to the end of protocol, mice were randomlydivided and intraperitoneally injected with SPX 29µg/kg (C-SPX andF-SPX) or PBS (CTR and FRD). Caloric intake and body weightwere recorded. At the end of protocol, plasma samples were collected and epididymal adipose tissue (EAT) was dissected, weightedand processed for qPCR and flow cytometry analysis. We founda signifcant negative correlation between body weight and bodyweight variation during SPX treatment in F-SPX and C-SPX. Glycaemia was unchanged, but plasma triglycerides were decreasedonly in F-SPX vs. FRD mice and were similar to CTR/C-SPX levels.F-SPX also had signifcantly less EAT mass, reaching values similarto CTR/C-SPX. mRNA expression of pro-inflammatory markers inEAT decreased in both groups treated with SPX vs. their counterparts. IL10, an anti-inflammatory marker, increased in F-SPX micereaching similar values to CTR/C-SPX. Flow cytometry of stromalvascular fraction from EAT showed that ly6C+ monocytes and M1macrophages were signifcantly decreased in SPX treated animalsvs. their counterparts. M2 macrophages were increased in F-SPX,restoring the population to normal values. In conclusion, SPX induced a decrease in the inflammatory markers and restored the anti-inflammatory profle in EAT upon FRD. Furthermore, the negativecorrelation between body weight and weight variation indicates apossible use of SPX as a therapy for obesity/MS. PICT2015-2352.