INVESTIGADORES
LAGORIO MarÍa Gabriela
congresos y reuniones científicas
Título:
Fluorescence in root eudates of soybean seedling is influeces by phosphate starvation
Autor/es:
PABLO YARYURA, MARIANA LEON, GABRIELA CORDON, M. GABRIELA LAGORIO, GERARDO RUBIO, NORMA KERBER, NORMA PUCHEU Y AUGUSTO GARCÍA
Lugar:
Rosario, Argentina
Reunión:
Congreso; XLII Reunión anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2006
Resumen:
CB-P85
FLUORESCENCE IN ROOT EXUDATES OF SOYBEAN
SEEDLING IS INFLUENCED BY PHOSPHATE
STARVATION
Yaryura, P.; León, M.; Cordon, G.; Lagorio, M.G.; Kerber, N:;
Pucheu, N.; García, A; Rubio, G.
Instituto de Investigaciones Bioquímicas y Fisiológicas (IBYFCONICET),
FAUBA; INQUIMAE-FCEN, UBA
E-mail: yaryura@agro.uba.ar
Phosphorus is one of the essential macronutrients in plants.
Phosphate starvation leads to changes in morphological,
physiological and biochemical processes. Some roots fluoresce
when irradiated with UV light. This trait has been useful as a
marker in genetic studies. We studied the influence of phosphate
starvation on the amount of root and seed exudates and leaves
fluorescence in two soybean genotypes (FN-4.85 and FN-4.10).
Plants were grown in hydroponic cultures. The fluorescence was
determined with a spectrofluorometer in 21 days-old plants. Our
results indicated that fluorescence emission (F420nm) of root
exudates was significantly higher (p<0.05) in FN 4.85 and
significantly lower in FN 410 (p<0.05) in phosphate deficient
plants. In seeds, fluorescence had a different maximum emission
band (F503nm) than root exudates, but was identical between
both genotypes. Fluorescence in leaves showed the same
emission spectrum (F420nm) of root exudates, but no effects of
phosphate limitation was detected. We also determined that the
fluorescence ratio (F684/F737) for chlorophyll emission did not
vary under phosphate starvation. This is an indication that growth
for 21 days under the latter condition had not yet induced
quali/quantitative changes in photosystems. These results could
reflect the genetic variability in soybean which could perhaps be
extended to other soybean genotypes.