INVESTIGADORES
SEDAN Daniela Yazmine
congresos y reuniones científicas
Título:
Microcystin-LR oral prolonged exposure at low doses induced hepatic and intestinal alteration in mice.
Autor/es:
SEDAN DANIELA; LAGUENS MARTIN; COPPARONI GUIDO; GIANNUZZI LEDA; ANDRINOLO DARÍO
Lugar:
Buenos Aires
Reunión:
Congreso; TIAFT. 52nd ANNUAL MEETING OF THE INTERNATIONAL ASSOCIATION OF FORENSIC TOXICOLOGISTS.; 2014
Institución organizadora:
TIAFT ARGENTINA
Resumen:
Aims: Injury caused by Microcystin-LR (MC-LR) prolonged intraperitoneal administration in animals was widely reported; however there are few studies on the effects of prolonged oral exposure to these toxins. Since the most important form of exposure to MC-LR is orally at low doses and for long periods, it is important to assess the real impact of MC-LR on health in these exposure conditions. The aim of this work was to study the toxic effects of MC-LR in a prolonged oral exposure to low doses in N: NIH-Swiss mice. Methods: MC-LR was purified from natural blooms of Microcystis aeruginosa and quantified by HPLC-DAD. Two groups of 6 mice were treated with 100 μgMC-LR/kg and 50 μgMC-LR/kg body weight by gavage every 48 h during 1 month. At the end of the study, liver, kidney, intestine, lung and heart histological studies (Hematoxylin & Eosin, Red Oil, PAS and Thrichrome stain) and toxin determination (phosphatase kit) was carried out. Alkaline phosphatase (ALP), Aspartate transaminase (AST), Alanine transaminase (ALT) were determinate on blood samples. Results and conclusions: Dose dependent cytoplasmatic microvacuolation with 15.3 ± 1.6 % and 3.6 ± 0.6 % of hepatic steatosis were observed in centrolobulillar zone of mice treated with 100 μgMC-LR/kg and 50 μgMC-LR/kg respectively. However, no changes were observed in ALT, AST and FAL plasma levels. Intestine samples have shown a significant dose-dependent decrease of intraepithelial lymphocytes: 28.7 ± 5.0% and 44.2 ± 8.7% for 50- and 100- μg MC-LR/kg treated animals, respectively. These results are consistent with tissues toxin levels. These results confirm that oral exposures to low doses of MC-LR, even when go unnoticed, generate damage not only in liver but also in intestine.
