CLEANER PRODUCTION: DESIGN OF NEW MEDIA FOR BIOTECHNOLOGICAL INDUSTRY

MORENO MARIA JULIETA; EXEQUIEL ELÍAS GONZALEZ; CABRERA CARLA AGOSTINA; DI TOTO BLESSING LILIAN EDITH; CERUSICO, NICOLAS ABEL; SESTO CABRAL M. EUGENIA

San Miguel de Tucuman

Congreso; XXXIV Jornadas de Biología de Tucuman; 2018

Asociación de Biología de Tucuman

Production costs are among the main factors in biotechnological production. The trend towards environmental sustainability and development of renewable resources has significantly increased interest in maximizing efficiency and minimizing costs and waste. Cleaner production is a strategy which will contribute to sustainable development.In this sense, our purpose was to design a non-waste process production line to reuse remnant biomass by transforming it into high added value products, using 4R technologies. Bacterial residual cells (BCR) were submitted to a lysis process by physical techniques in order to release their contents. Then, 20 media were designed by replacing 50% and 100% of proteins from commercial media. In order to define applicability of all new media, growth kinetics were tested at 600 nm with Lactobacillus plantarum, Lactobacillus alimentarius, Enterococcus faecium and Pseudomonas fluorescens. We tested growth kinetics by using MRS as broth for Lactic Acid Bacteria (LAB) and LB was used as broth for pseudomonas. Out of the media designed, 16 were equal to or better than 10%- 35% of the strains tested in the reference media. The usefulness of these growth media for the growth of probiotic strains and other strains of biotechnological interest allowed us to obtain a patent from INPI N°20170123114.