Contribution of Nrf2/Cav-1 association in the cellular senescence process during experimental pituitary tumour development

GRONDONA EZEQUIEL; SOSA LV; DE PAUL AL ; CARREÑO L; LUQUES N; TORRES AI; MONGI BRAGATO B; GUTIÉRREZ SILVINA.

Cordoba

Jornada; XIX Jornada de la Facultad de Ciencias Medicas (JIC); 2018

FCM, UNC

We have recently shown evidence of the emergence of cellular senescence as a mechanism responsible for pituitary proliferative arrest, whose onsetmay be favoured by metabolic alterations and oxidative stress. One of the antioxidant pathways that is activated in order to counteract the damagecaused by stress involves the erythroid nuclear factor 2 (Nrf2). In addition, caveolin-1 (Cav- 1), a structural protein of the caveolae, has also beenimplicated in the mechanisms of tumour growth control. In this context, the aim of this work was to analyze the possible association Nrf2/Cav-1 in thesenescent response during experimental pituitary tumour development.Tumour development was induced in adult male Wistar rats through subcutaneous incorporation of silastic capsules containing estradiol benzoate (30mg) for 10, 20, 40 y 60 days (E10-60, n=5). Control group: animals treated with empty capsules (n=5). Then, by Western Blot (WB), we analyzed Cav-1,Nrf2 and Keap1 (Nrf2 inhibitor) protein expression from cytoplasmic extracts and the Nrf2 expression was also evaluated in nuclear extracts. Tissue andsubcellular Nrf2 and Cav-1 distribution were analyzed by immunohistochemistry (IHC) in paraffin-embedded samples. Statistical Analysis: ANOVAFischertest (p