Alterations in the insulin pathway in the ovary of cows with induced follicular persistence

GAREIS N.; STASSI A.; RODRIGUEZ F.; CATTANEO ML.; SALVETTI N.; ORTEGA H.; HEIN G.; REY F.

Mar del Plata

Otro; LXIII Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2018

Sociedad Argentina de Investigación Clínica

Several local factors, including components of the insulin pathway, are involved in the ovarian follicular persistence associated with anovulation. In previous studies, we detected alterations of insulin-signaling intermediaries in the ovaries from cows with cystic ovarian disease. Therefore, we aimed to study the protein expression of IR, IRS1, PI3K, total and phosphorylated AKT by immunohistochemistry in ovarian follicular structures from a model of follicular persistence. We also measured the insulin concentrations in serum and follicular fluid by radioimmunoassay. The model was performed with an intravaginal progesterone device to get subluteal concentrations of progesterone, obtaining dominant follicles in the expected day of ovulation (n=5; P0) and follicles that persist for 5 (n=5; P 5), 10 (n=5; P10) or 15 days (n=5; P15) relative to the expected time of ovulation. Controls cows were ovariectomized in proestrus (n=5; C). Serum insulin concentrations were higher in all persistence groups than in control cows and the intrafollicular concentrations were higher in P5, P10 and P15 groups respect to the control group (p