DEVELOPMENT OF METABOLIC ALTERATIONS BY GESTATIONAL STRESS

BURGUEÑO, ADRIANA L

Mar del Plata

Congreso; LXIII Reunion Anual de la Sociedad Argentina de Investigacion Clinica; 2018

Prenatal exposure to stressmay program the fetal HPA axis, leading to altered metabolism in later life,associated with obesity and diabetes. This could be worsen if the individualdoesn?t eat healthy. We studied the effect of prenatal stress (PS) on metabolismand the impact of high fat diet (HFD). Pregnant BALB/c andC57BL/6J female mice were stressed during the last week of pregnancy. Offspringwere fed with HFD or a standard diet (SD). We observedthat PS only: increased cholesterol (CHOL) and triglycerides (TRI) levels inBALB/c males (p<0.001 vs NPS+SD), C57BL/6J males showed alterations in theinsulin sensitivity test (IST). When we added the HFD influence: PS+HFD malesof both strains and BALB/c females had a higher body weight than NPS+HFD and PS+SDgroups (p<0.001). In the IST, C57BL/6J mice showed alterations due to PStogether with HFD. Females BALB/c PS+HFD had increased TRI and CHOL (p<0.001vsNPS+HFD, p<0.05 vs PS+SD). Plasmatic TRI were also increased in C57BL/6Jmales (p<0.001 vs NPS+HFD and PS+SD). The gene expression in adipose tissueshowed a decrease in ADIPO in BALB/c and in females C57BL/6J of PS+SD(p<0.01 vs NPS+SD). FOXO1 increased in BALB/c PS+SD females (p<0.05 vs NPS+SD),while SIRT1 decreased in BALB/c PS+SD males (p<0.001 vs NPS+SD). BALB/cmales were more sensitive to developing metabolic alterations due to PS, BALB/cfemales PS showed an increase in body weight and plasma TRI. Males and femalesC57BL/6J developed metabolic alterations due to PS only after HFD feeding. Inboth strains females showed greater resistance to PS. The decrease in theexpression of ADIPO and SIRT1 in BALB/c males would indicate a lower insulinsensitivity, which may be detected earlier than at the physiological level. Thisleads us to propose these genes as possible premature markers of a futuremetabolic imbalance.