Effect of nitrative stress on placental AQP9 and its role in the pathogenesis of preeclampsia

MEDINA, YOLLYSETH; DI PAOLA, MAURICIO; REPPETTI, JULIETA; ANUD, CAROLINA; DAMIANO, ALICIA

Mar del Plata

Congreso; SAIC. SAI. SAFIS 2018; 2018

SAIC, SAI, SAFIS

Effect of nitrative stress on placental AQP9 and its role in the pathogenesis of preeclampsia.AbstractPreeclampsia is a gestational hypertensive syndrome of unknown etiology unique to humanpregnancy. Increased levels of reactive nitrogen species (RNS) were described in preeclampticplacentas. RNS promotes the formation of peroxynitrite (ONOO-), that covalently modify proteinsby nitration of tyrosine residues [3-nitrotyrosine (3NTp)] and lead to a non-functional protein.Nitration of a wide range of placental proteins were found increased in preeclampsia. Previously,in these placentas we reported an overexpression of AQP9 with a lack of functionality.Our aim was to evaluate the effect of nitrative stress mediated by ONOOon placental AQP9expression and functionality.This study was approved by the ethics committee of the Hospital Nacional Dr. Prof. A. Posadas.Explants from normal term placentas were cultured under conditions of nitrative stress induced by100 µM de ONOO-. The cell viability, cytotoxicity, and cell damage indexes were evaluated.AQP9 expression and water uptake were also studied. The amount of 3NTp-AQP9 in trophoblastfrom preeclamptic and normal patients was determined using an immunoprecipitation assay.In the presence of 100 µM of ONOO-, AQP9 protein expression significantly increased (n=7;p