Differences in endometrial mRNA expression of important genes related to fertility in repeat breeder and fertile cows

JAUREGUIBERRY M; MADOZ L; QUINTANA S; MARIN M; BURUCÚA M; TIZZANO M; RABAGLINO B; REARTE R; PECORARO M; DE LA SOTA R

Sapporo

Congreso; The 30th World Buiatrics Congress 2018 Sapporo; 2018

Organizing Committee for WBC 2018 Sapporo; The Japanese Society of Farm Animal Veterinary Medicine, Japan Veterinary Medical Association

Objectives An altered uterine environment produced by changes in the endometrial mRNA expression of key genes may be responsible for reduced fertility in repeat breeder cows (RBC). The aim of the present study was to compare the endometrial gene expression of epidermal growth factor receptor (EGFR), nodal growth differentiation factor (NODAL), prostaglandin-endoperoxide synthase 2 (PTGS2), estrogen receptor 1 (ESR1), and progesterone receptor (PGR) in RBC and fertile cows (FC) during diestrous by RT-qPCR. Materials and methodsThis study was conducted in commercial dairy farms located in Castelli (36º05?S, 57º48?W), Brandsen (35°10?S, 58°13?W) and Lobos (35º11´S, 59º05´W), province of Buenos Aires, Argentina. A total of 82 grazing Holstein dairy cows (RBC and FC) were evaluated to select the cows that were included in the study. We considered a RBC as a cow without any clinical disease that had ≥ 3 unsuccessful artificial insemination (AI), and a FC as a cow without any clinical disease and that became pregnant at first or second AI. Cows were evaluated by endometrial cytology to exclude cows with subclinical endometritis (SCE) and a blood sample from the coccygeal vein was obtained to exclude cows with