IFNb expression in the nervous and respiratory system of bovine alphaherpesviruses-infected calves

BURUCÚA M; QUINTANA S; ODEÓN A; PÉREZ S; MARIN M

Mar del Plata

Conferencia; REUNIÓN CONJUNTA SAIC SAI SAIF 2018; 2018

SAIC, SAI, SAIF

Induction of interferon (IFN) β is an important feature of bovine herpesviruses (BoHV) infections. The aim of this research was to determine the variations in IFNβ expression in the respiratory tract and nervous system of calves during BoHV-1 and BoHV-5 experimental acute infection [6 days post-infection (dpi), n=4] and reactivation (dexamethasone treatment, 25 dpi, n=4) in comparison to mock-infected animals (n=2). RNA was extracted from nervous system [olfactory, frontal and posterior cerebral cortex, cervical medulla and trigeminal ganglia (TG)] and respiratory tract (retropharyngeal lymph nodes, nasal mucosa, trachea and lung) to determinate the gene expression of IFNβ1 by RT-qPCR. Relative expression analysis was performed with REST software (P