INVESTIGADORES
RUMBO Martin
congresos y reuniones científicas
Título:
EXPRESSION OF PROINFLAMMATORY GENES IN INTESTINAL MUCOSA AS REJECTION PREDICTORS IN HUMAN INTESTINAL TRANSPLANTATION
Autor/es:
HERNÁN CAGNOLA; DOMINIK MEIER; AGUSTINA ZAMBERNARDI; CAROLINA RUMBO; GUILLERMO DOCENA; FERNANDO CHIRDO; GONDOLESI GABRIEL; MARTIN RUMBO
Lugar:
Viña del Mar, Chile.
Reunión:
Congreso; IX Congreso of the Latin American Association of Immunology; 2009
Resumen:
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Intestinal transplantation
is the accepted treatment for irreversible intestinal failure and complications
of total parenteral nutrition. Acute cellular rejection (ACR) is a frequent
complication, a first cause of graft loss and is usually diagnosed by
histological analysis. No markers to detect ACR are available.
Our aim was to characterize
the ACR process by gene expression analysis in intestinal mucosa along follow
up for selecting candidate markers of rejection.
Expression of IFN-g, CXCL10,
CXCL11, CXCR3, and CCL20 was measured by RT-qPCR in graft biopsies obtained
during the first 3 months post-transplantation. Twenty-one
samples of 6 pediatric patients were analyzed, including 3 ACR
events, ranging from mild to severe. In samples taken during ACR, induction of
IFN-g (fold
increase range (FIR) 4 to 17, depending on individual sample), CXCL11 (FIR 8 to
22), CXCL10 (FIR 5 to 27), and CCL20 (FIR 2 to 22) was observed. Induction
levels correlated with the severity of
the rejection episode. IFN-g, CXCL10,
and CXCL11 expression levels increased before ACR indicating that these genes
might be putative predictors of rejection in the clinics.
Although a wider set of
analysis is necessary, our results demonstrate that graft gene expression
analysis is a valuable tool for ACR characterization.
