INVESTIGADORES
RUBINSTEIN Marcelo
congresos y reuniones científicas
Título:
REGULATION OF HYPOTHALAMIC EXPRESSION OFTHE PROOPIOMELANOCORTIN GENE BY NUCLEAR RECEPTOR FACTORS
Autor/es:
DE SOUSA, FJS; RUBINSTEIN M
Lugar:
San Miguel de Tucumán
Reunión:
Congreso; XLV Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2009
Institución organizadora:
SAIB
Resumen:
The proopiomelanocortin (POMC) gene encodes a prohormone
expressed mainly in the pituitary and the arcuate nucleus of the
hypothalamus. POMC-derived peptides play important roles in
responding to stress and controlling food intake and energy
balance. We have identified two enhancers - named nPE1 and nPE2
- that control POMC expression in the mammalian hypothalamus.
To identify transcription factors that might control POMCproopiomelanocortin (POMC) gene encodes a prohormone
expressed mainly in the pituitary and the arcuate nucleus of the
hypothalamus. POMC-derived peptides play important roles in
responding to stress and controlling food intake and energy
balance. We have identified two enhancers - named nPE1 and nPE2
- that control POMC expression in the mammalian hypothalamus.
To identify transcription factors that might control POMCPOMC expression in the mammalian hypothalamus.
To identify transcription factors that might control POMCPOMC
expression, we performed an one-hybrid screening with nPE2 as
bait and identified factors of the nuclear receptor superfamily that
can bind to an element in nPE2 that follows the consensus binding
site for these factors. In addition, this element is conserved in all
mammals and is present within a crucial region for nPE2 enhancer
activity. Since the factors we identified by one-hybrid screening are
not co-expressed with POMC in the brain, we sought to find other
factors based on the literature. One candidate is oestrogen receptorPOMC in the brain, we sought to find other
factors based on the literature. One candidate is oestrogen receptor
a (ERa) which is known to be involved in the control of POMC(ERa) which is known to be involved in the control of POMC
expression and energy balance, and is widely expressed in the
brain. By immunohistochemistry using a transgenic mouse model,
we found that around 30% of POMC-EGFP neurons co-express
ERa, indicating that this transcription factor is a strong candidate
for regulating the expression of the POMC gene. Further functional
studies are needed to corroborate this hypothesis.a, indicating that this transcription factor is a strong candidate
for regulating the expression of the POMC gene. Further functional
studies are needed to corroborate this hypothesis.POMC gene. Further functional
studies are needed to corroborate this hypothesis.