Optimization of direct shoot regeneration using mature zygotic embryos explants from Pinus elliottii var. elliottii x Pinus caribaea var. hondurensis

LUNA CLAUDIA; RIZA DAIANA; AYALA LILIAN PATRICIA E.; SANSBERRO PEDRO

Lavras

Simposio; 7th International Symposium on Production and Establishment of Micropropagated Plants; 2017

International Society for Horticultural Science

The hybrid pine isan economically important alternative for forest plantation in the northeast ofArgentine. However, some problems associated with the production of either F1or F2 seed production limits the rapid expansion of cultivation areas. Tissue culturemethodology was successfully applied to find the combination of seedstratification, basal medium and 6-benzyladenine (BA) concentration for inducing the largest number of shoots from regenerativeembryos explants. Vegetative buds were originatedthrough a direct pathway from mature zygotic embryos stratified at 4 °C (for 1to 6 days) and cultured on Murashige and Skoog (either half full strength)semisolid medium containing BA 0.1-5 mg∙L-1. The cultures wereincubated in a growth room at 27±2°C with a 14-h photoperiod (180 μmol∙m−2∙s−1PPFD,from fluorescent lamps) for 30 days.The regeneration frequency(93±2.5%) and the number of buds formed per responsive explants (13,2±8,2) wasgreater with explants stratified at 4 °C for 3 days and cultured on Murashigeand Skoog half strength medium plus BA 3 mg·L-1. In general, bothparameters diminished due to a higher concentration of either the basal mediumor BA.These methodologies can beused in breeding programs for the propagation and cryopreservation ofgermplasms.