Altering microtubule polymerization in Sertoli cells strongly affects their lipid metabolism

AVELDAÑO, MI.; PICARDI, MV; VALLES, AS; FURLAND, N.E

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

SAIB

ALTERING MICROTUBULE POLYMERIZATION IN SERTOLI CELLS STRONGLY AFFECTS THEIR LIPID METABOLISM.María Victoria Picardi, Natalia Edith Furland, Marta Isabel Aveldaño, Ana Sofía Vallés.Instituto de Investigaciones Bioquímicas de Bahía Blanca(INIBIBB)The aim of this work was to study the biological consequences of altering microtubule polymerization in Sertoli cells, with a special focus on their lipid metabolism. For this purpose, the effect of longterm exposures to nocodazole (NCZ) (0.01 µM) was studied on TM4 cells in culture. In addition to the expected effect of NCZ on the depolymerization of tubulin microtubules, a remarkable reorganization was observed in the intermediate filament network (vimentin), which showed a more peripheral distribution than in untreated cells. As compared to cells cultured in control conditions, those exposed to NCZ displayed an increased mitochondrial membrane depolarization (p