NONYLPHENOL INDUCES CYTOSKELETAL CHANGES AND RELEASE OF PROINFLAMMATORY MEDIATORS IN RAT SERTOLI CELLS IN VITRO

VALLES, A.S.; GODOY SEPÚLVEDA, R.; ORESTI, G. M.; MORENO, R.D.; AVELDAÑO, M.I.; REYES, J.G.

Buenos Aires

Congreso; SAIB; 2017

SAIB

Nonylphenol (NP), an alkylphenol present in plasticizers, is an endocrine disrupting chemical that is potentially dangerous for male reproduction in mammals including man. Because Sertoli cells (SC) provide structural and metabolic support to germ cells, in this study the hypothesis that exposures of SC to NP affect their metabolic functions and the production of bioactive molecules was evaluated. Primary cultures of SC were preincubated with [3H]arachidonic acid (AA) to label their lipids and then were treated with NP. The NP exposures resulted in increased concentrations of free AA in cells and medium, indicating that such AA was released from [3H]-labeled lipids (p