Diacylglycerol lipase activity in rod outer segments depends on the illumination state of the retina

CHAMORRO AGUIRRE, E.; GAVEGLIO, V.L.; PASQUARE SJ; GIUSTO, N.M.

Ciudad Autónoma de Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

The aim of the present research was to determine how the synthesis of endocannabinoid 2-arachidonoylglycerol (2-AG) for diacylglycerol lipase (DAGL) enzyme is modulated by the illumination state of the retina. DAGL activity was analyzed in purified rod outer segments (ROS) obtained from dark or light adapted retinas. Retinas were dissected from the eyes after dark or light adaptation. Dark-adapted bovine ROS (DROS) and bleached ROS (LROS) were purified by a discontinuous gradient of sucrose from retinas whose optic cup was either maintained under dim red light or exposed to light (3000 luxes) for 30 min. This activity was assayed using [3H] glycerol-DAG as substrate and determined by [3H]-MAG production. MAG was partially metabolized to glycerol by MAGL action. DAGL activity in LROS was higher than in DROS under all conditions assayed. When the enzyme activity was assayed in a range between 100 µg and 200 µg of ROS protein, [3H]-MAG production was increased in LROS (p