n6 dietary fatty acids excess does not affect reproductive success; however, it alters fetal development and its vitality

BIANCONI S; SOLÍS MR; SANTILLÁN ME; MOLINA RE; STUTZ G

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

SAIC, SAIB, SAI, SAFE, SAA, SAB, SAFE, SAFIS, SAH, SAP

Parents? feeding has significant impact on the fetus and its postnatal life. Essential fatty acids (EFA) n6 and n3 play a wide variety of roles in reproduction and development. Objective: to evaluate the influence of different dietary n6/n3 ratios on reproductive success (RS), placental and fetal development. Female adult mice fed 25 days with control diet (C,n6/n3=19.1,n=8), SF (C with 10% sunflower oil, n6/n3=87.2,n=8), F (C with 10% fish oil, n6/n3=1.3,n=8) and B (balanced, C with SF-5%- and F-3%-, n6/n3=5.2,n=8). Body weight and food intake were daily recorded.On day 25 they were placed with 2 males until vaginal plugs were detected (gestational day 0.5=GD0.5). On GD16.5 dams were weighed (g) and the following weights were also registered (g): uterus(UW), ovaries (OW), fetal-placental units (FPUW), fetuses (FW) and placentas (PW). RS was calculated. Number of total fetuses (TFN), normal (NF) and reabsorbed fetuses (RF), fetal length (cm), fetal vitality (FV), placental diameter (PD, mm) and fetal macroscopic characteristics (FMC) were evaluated. Statistics: ANOVA, Chisquare, Kruskall-Wallis. There were no significant differences in final dams? weight or RS. OW in SF and F were lighter than C (SF=0.013±8.2-4 and F=0.013 ± 9.9-4 vs. C=0.016±3.7-4; p