Apoptosis and host-defense peptide cathelicidins determine different outcomes of bovine alpha-herpesviruses neuropathogenesis

MARIN, M; BURUCÚA, M; RENSETTI, D; ODEÓN, A; COBO, E; QUINTANA, S; PEREZ, S. E

París

Congreso; International Society of Neurochemistry (ISN) and the European Society for Neurochemistry Meeting; 2017

International Society of Neurochemistry (ISN) and the European Society for Neurochemistry

Alpha-herpesviruses are closely related viruses that cause neuro¬logical dis¬ease in humans and cattle. Bovine herpesvirus (BoHV) type 5 is an important cause of encephalitis in cattle. How¬ever, encephalitis by BoHV-1 occurs occasionally. It is unknown how the innate immune response contributes to their differences in neuropathogenesis. BoHV-5 specifically induced the expression of Toll-like receptors (TLRs) in bovine neural inflamed tissue (Marin et al., 2014). Antimicrobial cathelicidin peptides modulate TLR4 expression in epithelial cells although their function in the nervous system remains elusive. These innate factors together with specific BoHV-apoptotic potential could be determinant in the neuro¬patho¬genesis as they could promote or limit the inflammatory response. In this study, we determined apoptosis and the expression of cathelicidins in the bovine nervous system during BoHV-1 and 5 acute infections. Calves were inoculated with BoHV-1 Cooper or BoHV-5 97/613 strains (106.3 TCID50) or inert culture medium (control). At 6 days post-infection (dpi), different regions of central nervous system (CNS) and trigeminal ganglion (TG) were collected for immunocytochemical detection of cleaved caspase 3 and messenger gene determination of bovine cathelicidins BMAP27 and BMAP28 (RT-qPCR). At 6 dpi, caspase 3-apoptotic neurons were detected in the TG of BoHV-1-infected calves, whereas fewer numbers of caspase 3 positive neurons were observed in BoHV-5-infected calves. Cathelicidins expression was up- and down-regulated in CNS from BoHV-1- and BoHV-5-infected calves, respectively. BMAP27 was noticeably up-regulated in TG from BoHV-1-infected calves, while BMAP28 was only detected in BoHV-5-infected calves. Our find-ings sug¬gest that modulation of apoptosis and cathelicidin expression orchestrate the final in-flam¬matory outcomes of alpha-herpesviruses infection of the nervous system. Inhibition of both factors might be responsible for neuro¬logical lesions in BoHV-5 infection. Further research will be required to determine the role of cathelicidins during infectious diseases of the CNS.