Signal anticipation in a GPCR pathway—Single cell measurements of scaffold recruitment show rapid does response alignment before receptor binding reaches equilibrium

ARIEL CHERNOMORETZ; ALAN BUSH; PABLO BALENZUELA; LUCIANA BRUNO; RODRIGO LAJE; TY THOMSON; ROGER BRENT; ALEJANDRO COLMAN-LERNER

Okinawa, Japón

Workshop; Workshop de Biología de Sistemas de rutas de transducción de señales de MAP kinasasa en levaduras y células de mamífero; 2008

Okinawa Institute of Science and Technology

Cells implement precise responses to signals. We investigated in S.cerevisiae early pheromone signal propagation. Using microscope-based cytometry, we measured membrane recruitment of the scaffold protein Ste5. Ste5 is recruited in a few seconds in response to high pheromone. We found that pheromone dose information is transmitted precisely to Ste5 but that recruitment reaches plateau levels faster than pheromone:receptor complex ("signal anticipation"). In this talk we describe a simplified model of the complex biochemical reaction network and explore what feature of the model could explain the observed signal anticipation effect.