The protective effect of lipoic acid against oxidative damage of brain structures in a glaucoma rat model

FERREIRA SANDRA MARIA; REIDES CLAUDIA; HVOZDA ARANA AILEN; PEVERINI AGUSTINA; JANEZIC NATASHA; LASAGNI VITAR ROMINA; LERNER FABIAN; LLESUY SUSANA

Congreso; Congress of the European Society of Ophthalmology (SOE 2017); 2017

SOE

Purpose: Evidence of oxidative process was found in glaucoma brain so the use of an antioxidant therapy may hold a promise for treatment. The aim of this study was to evaluate the protective effect of lipoic acid (LA) against oxidative damage of geniculate nucleus (GN)and visual cortex (VC) in an experimental glaucoma model.Method: Wistar rats (3 months) were divided in four groups: glaucoma in which rats were operated under a microscope by cauterized two of the episcleral veins (G), glaucoma treated with LA 100 mg/kg i.p. (LG), control which received a sham procedure (C) and control treated with lipoic acid 100 mg/kg i.p. (LC). Seven days after surgery rats were euthanized, brains were removed and GN and VC were separated. Thioredoxin reductase (TRxR),glutathione reductase (GR) and uperoxide dismutase (SOD) activities, protein oxidation (PO), damage to lipids (TBARS) and glutathione (GSH) were evaluated.Results: Comparing LG group to G group: TRxR increased 52% in GN (G:7.6±0.4nmol/min.mg protein p< 0.01) and 26% in VC (G:9.9±1.0 nmol/min.mg protein p< 0.05), GR increased 82% in GN (G:8.2±1.2 nmol/min.mg protein p< 0.01) and 300% in VC (G:5.1±1.3 nmol/min.mg protein p< 0.001), SOD increased 23% in GN G:18.0±1.1 U/mg protein p