An early antioxidant response is generated by human conjunctival epithelial cells after diesel exhaust particles (DEP) exposure

JANEZIC, NATASHA; LASAGNI VITAR ROMINA; TAU JULIA; HVOZDA ARANA, AILEN GALA; TESONE AGUSTINA; PEVERINI AGUSTINA; REIDES, CLAUDIA; BERRA ALEJANDRO; FERREIRA SANDRA MARIA; LLESUY, SUSANA

Mar del Plata

Congreso; LXI Reunion Cientifica Anual de la SAIC; 2016

Sociedad Argentina de Investigación Clínica

The exposure to airborne particulate matter shows adversehealth effects in urban population. The aim of the present studywas to evaluate oxidative stress markers in human conjunctivalepithelial cells (IOBA-NHC) after the incubation with diesel exhaustparticles (DEP) for 1 hour. The incubation was performed with DEPat concentrations of 50 and 100 μg/mL for 1 hour and the followingparameters were evaluated: glutathione S-transferase (GST), glutathionereductase (GR), thioredoxin reductase (TrxR) and glucose6P-deshidrogenase (G6PDH) activity; reduced glutathione (GSH);lipid and protein oxidation. One-way ANOVA test and Dunnett?stest as post hoc test were used for statistical analysis. The cells exposedto 50 and 100 μg/mL of DEP showed a significant increasein the activity of GST (74% and 99%, respectively, p