Changes in the brain redox status in a glutamate excitotoxicity model

HVOZDA ARANA, AILEN GALA; JANEZIC, NATASHA; PEVERINI, AGUSTINA; REIDES, CLAUDIA; LASAGNI VITAR, ROMINA; WEISCHLER, NATALIE; LLESUY, SUSANA; FERREIRA, SANDRA

Mar del Plata

Congreso; LXI Reunion Cientifica Anual de la SAIC; 2016

Sociedad Argentina de Investigación Clínica

Glutamate is an excitatory neurotransmitter in central nervoussystem. Glutamate excitotoxicity is thought to play an importantrole in neuronal damage. The aim was to evaluate changes in redoxhomeostasis in brain of rats subject to an experimental modelof glutamate excitotoxicity.The experimental model consisted ofone group (n=6) injected ip with 1g glutamate/kg weight (GG) atdays 1, 5 and 9 and control group (CG) injected with saline solution(n=6) at same days. The following markers were evaluated inbrain homogenates: the activities of superoxide dismutase (SOD),catalase (CAT) thioredoxin reductase (TR), glutathione peroxidase(GPx), glutathione transferase (GT) glutathione reductase (GR),and glucose-6-phosphate dehydrogenase (G6P), protein oxidation(PO), lipid damage (TBARS), ascorbic acid concentration (AA), andglutathione (GSH) levels. SOD activity was 5.90±0.10 U/mg proteinin GG (CG 4.63±0.17 U/mg protein p