Doxorubicin loaded magnetic nanotheranostic: Evaluation on the loading capability, their retention during drug targeting and in vivo toxicity

P. AZCONA; D. IGARTÚA; S. DEL VALLE ALONSO; M. J. PRIETO; M. GRÜNHUT; V. LASSALLE

Bahia Blanca

Conferencia; International Conference on Science and Technology of Composite Materials (COMAT); 2018

The goal of this work is to evaluate the effect of different reaction parameters on the adsorption of Doxorubicin on magnetitenanoparticles modified with folic acid (MNPs@FA). Three nominal Doxo/MNPs@FA mass ratios, the influence of pH and theincubation time were evaluated to determine the set of parameters ensuring the best formulation regarding not only the drugloading capability, but also its physicochemical properties. All the obtained samples were exhaustively characterized by FTIR, UV-Vand atomic absorption (AA) spectroscopy, dynamic light scattering, z potential and TEM. A novel continuous flow system basedsystem was designed to estimate their retention ability in an environment simulating the bloodstream under the influence of anexternal magnet. This represents a reliable approximation to infer the amount of nanotheranostic able to reach the target site.Besides, in vivo assays were developed on Zebrafish larvae (Danio rerio) model to assess the toxicity of nanotheranostics.