Changes of hippocampal oxidative status in immature rats exposed to noise: in

MOLINA, SJ; CAPANI, F; GUELMAN, LR

Washington, DC

Congreso; 47th Annual Meeting of the Society for Neuroscience (SFN), Neurosciencie 2017.; 2017

Society for Neuroscience

Previous studies of our laboratory showed that exposure of immature rats tomoderate noise can induce hippocampus (HC)-related behavioral, biochemicaland histological alterations during the peri-adolescence period. In addition,rearing animals in an enriched environment (EE) has shown to be e􀃗ective inthe reversal of most behavioral alterations. However, comparative data of HCoxidative levels at di􀃗erent post-exposure ages were not obtained yet. Thus,the aim of the present work was to test the potential di􀃗erences in HCoxidative status of rats exposed to noise at an early developmental age as wellas the possible reversal of these changes by rearing in an EE for di􀃗erentperiods. Male Wistar rats of 7 days were exposed for 2 hours to white noise(95-97 dB). After weaning, groups of 3-4 rats were transferred to an enrichedcage, consisting of di􀃗erent toys, a wheel, plastic tunnels and ramps. Othergroups were placed in standard cages. One or two weeks later, levels of Trx1 -an antioxidant of the thioredoxin family- were tested through Western blotexperiments. Results showed that Trx-1 levels were increased in exposedanimals one week after weaning, when compared with non-exposed rats.However, rearing animals in an EE was e􀃗ective in restoring these di􀃗erences.In contrast, when Trx-1 levels were tested two weeks after weaning, there wereno signi