PERSONAL DE APOYO
ELESGARAY Rosana
congresos y reuniones científicas
Título:
Renal oxidative stress response to atrial natriuretic peptide in hypertension.
Autor/es:
COSTA MARÍA ÁNGELES; ROMERO MARIANA; CANIFFI CAROLINA; BOUCHET GONZALO; MENDES GARRIDO FACUNDO; ELESGARAY ROSANA; TOMAT ANALÍA; ARRANZ CRISTINA
Reunión:
Congreso; Twentieth European Meeting of Hypertension, European Society of Hypertension.; 2010
Resumen:
The precise
mechanisms leading to the pathogenesis of essential hypertension and renal
damage remain unknown. Spontaneously hypertensive rats (SHR) is a model of
hypertension which is known to be associates to different risk factors that
ends in cardiovascular and renal disease. In previous
studies we demonstrated that atrial natriuretic peptide (ANP) infusion
increases nitric oxide synthase activity in kidney of SHR, but how ANP can
modify oxidative stress in this model of hypertension has not been studied yet.
The aim was
to investigate the effects of chronic infusion with ANP on renal oxidative
stress in SHR.
Methods: 8
weeks-old SHR and Wistar Kyoto (WKY normotensive rats) were infused (14 days,
subcutaneous osmotic pumps) with ANP (100 ng/hr/rat) or saline (S). After
experimental period, we determined in kidney: thiobarbituric acid-reactive
substances (TBARS, nmol/mg protein), glutathione concentration (mg/mg protein),
and the activity of: glutathione peroxidase (GPx, pmol/min.mg protein),
catalase (CAT, pmol/mg protein) and superoxide dismutase (SOD, USOD/mg
protein).
Results:
TBARS
Glutathione
GPx
CAT
SOD
WKY S
0,113±0,015
0,271±0,038
181,1±19,4
0,847±0,102
7,691±0,505
WKY ANP
0,098±0,010
0,398±0,028*
180,3±21,2
0,956±0,098
8,021±1,654
SHR S
0,162±0,025*
0,178±0,046*
243,1±22,1*
0,951±0,105
7,098±0,653
SHR ANP
0,082±0,009#
0,374±0,027#
303,2±30,3#
1,101±0,142
8,452±0,779
*p< 0,01 vs WKY S;
#p<0,01 vs SHR S.
SHR
shown higher levels of TBARS and GPx activity than WKY rats. Renal glutathione
concentration was lower in hypertensive rats than in normotensive ones. CAT and
SOD basal activities were similar in both groups and ANP treatment induced no
changes their activities. In WKY rats, ANP treatment increased glutathione
concentration. In hypertensive rats, ANP chronic infusion diminished TBARS and
increased glutathione renal concentration and GPx activity, indicating a
reduction in renal oxidative stress.
Conclusion:
Chronic treatment with ANP improved, almost in part, renal oxidative damage in
this model of hypertension in rat.