Group II metabotropic glutamate receptor activation prevents apoptotic-like death cultured astrocytes induced by nitric oxide

D. DURAND; C. CARUSO; M. LASAGA

Búzios, RJ, Brazil

Congreso; Congreso of Neurosciences of Latin America, Caribbean and Iberian Peninsula; 2008

IBRO/LARC

We investigated the effect of (-)2-oxa-4-aminocyclo-[3.1.0] hexane-4,6-dicarboxylic acid (Ly379268) and (2S,1´S,2´S)-2-(carboxycyclopropyl)glycine (L-CCG-I), selective agonists of group II metabotropic glutamate receptors (mGluRs), on nitric oxide (NO)-induced cell death of rat astrocytes in culture. Astrocytes were incubated for 48 h. with diethylenetriamine nitric oxide adduct (DETA/NO) 1 mM in presence or absence of Ly 379268 100 mM or L-CCG-I 1 mM. Receptor selectivity was verified with a specific group II mGluR antagonist, (2S)- -ethylglutamic acid (EGLU) at 300 mM. Astrocytes viability was reduced significantly by DETA/NO by 35% (p< 0.01 vs. control), as measured by MTT method, while both Ly 379268 and L-CCG-I prevented this effect (p