EFFECT OF ALLOPREGNANOLONE, A PROGESTERONE METABOLITE, OVER HUMAN OVARIAN CANCER CELL LINES PROGRESSION STEPS: POTENTIAL USE AS THERAPEUTIC TOOL.

LACONI MR; RODRIGUEZ CE; SANHUEZA MA; PELEGRINA LT; CÁCERES ARR

Mar del Plata

Congreso; Reunión conjunta de sociedades de biociencias; 2017

Ovarian cancer is one of the most common cause of gynecologiccancer death. Allopregnanolone (ALL), a progesterone (P4) metabolite, modiÀes ovarian physi-pathological processes. Changes inALL levels during cycle or under stress situations can conduce toalterations in ovarian development. This was the Àrst evidence thatALL induces ovarian morpho-physiological changes altering proliferation, apoptosis and angiogenesis, crucial for cancer progression.Epidemiologic and in vitro studies have shown controversial dataabout P4 in cancer. The effect of P4 metabolites over ovarian cancer is relevant and require more in-deep trials due to it could beinvolved on cancer progression. The hypothesis is that changes inALL concentrations affect ovarian tumor progression. We investigated proliferation, apoptosis, clonogenic capacity and migration ofhuman ovarian cancer cell lines IGROV-1 and SKOV-3. Cell lineswere exposed to a range of P4 and ALL concentrations (10-11-10-5 M)for 72 h. We showed that ALL increased proliferation in a concentration dependent manner by MTT, with a maximum effect of 44.5± 13.5 % on IGROV-1 (p