PATERNAL MODERATE ALCOHOL CONSUMPTION MODIFIES MOUSE PERI-IMPLANTATION EMBRYO DEVELOPMENT

LUCILA GOTFRYD; FERNANDO FUENTES; MELISA CELESTE SANCHEZ; GABRIELA SALAMONE; JUAN CARLOS CALVO; ELISA CEBRAL; VANINA FONTANA

Congreso; Reunión conjunta de sociedades de biociencias; 2017

Previously, we showed that paternal alcohol intake partially inhibits sperm capacitation, increases nuclear decondensation rate and deregulates the dynamics of pronucleus formation and fertilization. Aim: To evaluate the effect of paternal alcohol consumption and its consequences on mouse early embryo development focusing on trophoblast (TB) differentiation and inner cell mass (ICM) as critical embryo stages invading maternal decidua. Methods: CF-1 fertile males were exposed (treated group, T) or not (control group, C) to 15% (v/v) ethanol in drinking water ad libitum for 15 days. They were mated with a fertile nontreated CF-1 female (1:1). Positive mating females were sacrificed at day 2 of gestation to obtain 2 cell embryos which, then, were cultured for 7 days. Embryo differentiation, growth and morphology were evaluated during pre- and peri-implantation phases in vitro. Then, embryos were classified as type A (ICM: protruding aggregates of compact cells; TB: symmetric monolayer of flat and elongated cells) or type B (ICM: disaggregated, few scattered or no cells, TB: asymmetric trophoblast outgrowth). Frequency differences between C and T were tested by Fisher´s exact test. Results: Male alcohol consumption for 15 days delayed the embryo differentiation and growth by detention/fragmentation and altered the embryo morphology. Differences between type A and B distribution in C and T were statistically significant for ICM and TB in both groups. For ICM: Type A 53% C vs 10% T (p