Strategy to study parameters able to predict longevity in medfly populations

BOCCHICCHIO P; PUJOL-LEREIS L; ROSSI F; TURDERA L; PÉREZ M; RABOSSI A; QUESADA-ALLUÉ L

Mendoza

Congreso; LII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2016

The Medfly (C.capitata) is the main orchards world pest. Functional Senescence refers to real physiological output, eventually leading to different mortality rate. To predict changes in longevity dependent on genetic microheterogeneity of Medfly lab populations subjected or not to stress we worked out both lethal and non-lethal assays. To establish a parameter reflecting different homeostaticequilibria, we analyzed quantitative changes of 38 Lipids categories, further reduced to significant 25 ones. Thus giving rise to Principal Components for each sex and body parts. Higher temperature of growing, chill coma induction or oxidative stress induced by Hematoporphyrin were the main factors studied. These and other biochemical and gene expression parameters were correlated with non lethal estimations of behavioral output like mobility and RING (negative geotaxis) experiments. Looking further for longevity predictors we analyzed spontaneous and/or fight-induced supine events during the early adulthood of medflies. We demonstrated a correlation between the rate of supine events and longevity that also roughly correlated with changes in biochemical and gene expression parameters. Most important, supine rate also correlated with other non-lethal assays like RING. Age- and stress-dependentchanges in a peculiar immune reaction were also explored