MODULATION OF OSTEOCLAST DIFFERENTIATION BY PLACENTAL CYTOQUINES

CUSTIDIANO ANDREA; SACERDOTI FLAVIA; DE LEON ROMINA; GENTILE TERESA; CANELLADA ANDREA

BUENOS AIRES

Congreso; FIST FRENCH-ARGENTINE IMMUNOLOGY CONGRESS (FAIC) , LVII REUNION ANUAL DE LA SOCIEDAD ARGENTINA DE INMUNOLOGÍA (SAI) , XII JORNADA CIENTÍFICA DEL GRUPO RIOPLATENSE DE CITOMETRÍA DE FLUJO 3 JORNADAS DE INMUNODEFICIENCIAS PRIMARIAS (SAP); 2010

During rheumatoid arthritis, macrophages differentiate into osteoclasts and bone destnuction occurs. We demonstrated that extracts from mouse placenta (EPs}, containing Th1 and Th2 cytokines; regulate the osteoclast differentiation of RAW 264.7 macrophages. Now we analyze, whether IL-10, TGF-beta, as well as the NFATc1 transcription factor are involved in such effect. Placenta! extracts were obtained at 7 days of pregnancy (EP7). RAW cells were cultured with RANKL and M-CSF (RM) in the presence or absence of EP7, and neutralizing antibodies (NA) against IL-10 and TGFbeta; the differentiation of the cells was determined by assessing multinucleated, tartrate resisten! acid phosphatase positive cells (TRAP+), using a commercial kit; and matrix metalloprotease (MMP) activity by zymogram. The effect of EP7 on NFATc1 expression /phosphorylation was assessed by westem blot. EP7 inhibited the RM-induced MMP activity and TRAP+ (inhibition vs RM: 68%, p