CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Lysophosphatidic acid participation during implantation in the rat
Autor/es:
SORDELLI MS; FARINA MG; CELLA M; FRANCHI AM; RIBEIRO ML
Lugar:
Rio de Janeiro, Brasil
Reunión:
Simposio; 2nd Symposium on Reproductive Immunology; 2009
Resumen:
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Introduction: During the last years it has become clear
that lipid molecules are active mediators of embryo invasion and that they play
pivotal roles in immune regulation. Knock out mice in LPA3, one of lysophosphatidic
acid (LPA) receptors, present severe deficiencies in implantation. LPA
modulates prostaglandins (PGs) production in bovine, pig and ovine uteri. PGs
and anandamide are described as crucial mediators of embryo invasion. PGE2 and
PGI2 promote neovascularization and decidualization in rodent uteri. High
anandamide levels correlate with aberrant implantation and blastocyst
apoptosis. Thus, the aim of our work was to study the effect of LPA on relevant
mediators of implantation in the rat.
Materials and methods: Day 5 pregnant rat uteri were incubated
with LPA 50µM for 6h. PGE2 production was assessed by radioimmunoassay and mRNA
and protein expression by RT-PCR and western blot. Significance was determined
using t-Student.
Results: The expression of LPA3 mRNA and protein
in the rat uterus was modulated during early gestation. The incubation with LPA
augmented FAAH expression (p<0.05), the enzyme that degrades AEA. LPA
treatment also increased COX-2 expression (p<0.05) and PGE2 synthesis
(2,1±0,1 vs 2,7±0,2 pgPGE2/mg ww, p<0.05) . Finally, LPA was capable to
induce the expression of IGFBP-1, a molecular marker of decidualization.
Conclusion: LPA is a potent lipid mediator that seemed
to favor embryo implantation.