HEXACLHOROBENZENE STIMULATES VASCULAR ENDOTHELIAL GROWTH FACTOR SECRETION IN THE HUMAN BREAST CANCER CELL LINE MCF-7, PROMOTING THE ANGIOGENIC SWITCH

PONTILLO, CAROLINA; ALEJANDRO ESPAÑOL; MIRET NOELIA; CHIAPPINI FLORENCIA; COCCA CLAUDIA; ALVAREZ L; KLEIMAN, D; MARÍA E. SALES; RANDI, ANDREA

Congreso; LXI reunion anual de la sociedad argentina de investigación clínica; 2016

Hexachlorobenzene (HCB) is an organochlorine compound found in maternal milk and in lipid foods. Chlorpyrifos (CPF) is an organophosphate pesticide used for pest control in agriculture. Breast cancer is by far the most frequently diagnosed cancer in women. HCB and CPF act as endocrine disruptors stimulating cell proliferation in different breast cancer models. Vascular endothelial growth factor (VEGF) and cyclooxygenase-2 (COX-2) promote tumor proliferation and angiogenesis. Nitric oxide (NO) and abnormal expression of nitric oxide synthases (NOS1, NOS2, NOS3) are implicated in cancer biology and tumor progression. We examined the action of HCB and CPF on angiogenesis in mammary carcinogenesis in vivo and in vitro. In a xenograft model with MCF-7(+ERα), HCB (3 mg/kg bw) and CPF (0.1 mg/kg bw) stimulated angiogenic switch (number of vessels/mm2) (HCB p