ATPase activities in muscle the euryhaline crab Chasmagnathus granulatus from Mar Chiquita coastal lagoon: differential response to emersion

PINONI,S; LÓPEZ MAÑANES, A,A.

Huerta Grande, Córdoba

Jornada; First Meeting of Argentine Biology Societies; 2007

We have shown the occurence in chela muscle of C. granulatus of ATPase activities with differential response to salinity. The aim of this work was to determine the response to emersion of Na+K+ATPase (NKA) and Na+ATPase (NA) sensitive (FS) and insensitive to furosemide (FI) activities in chela muscle. Male adult crabs acclimated at 10‰ and 35‰ salinity (S) were totally submerged for 24h (t0) and then exposed to air up to 240min. ATPase activities were determined at 15, 30, 60, 120 and 240min in homogenates (0.25MSucrose/0.5mMEGTA-Tris pH7.4) by measuring (13mM) ATP hydrolysis in (mM): 20imidazole (pH7.4) / 100NaCl /0.5EGTA. NKA: was determined as the difference between total (30KCl) and basal activity (without KCl/1 ouabain). NA activity was determined in the presence of 1ouabain/1NaN3, with (NA-FI) or without 2 furosemide. The difference of both assays represents the NA-FS activity. Five independent experiments were carried out. ANOVA was used as statistical analysis (p<0.05). After 240 min NA-FI activity increased in 35‰S (t0=20.8±5.9 and t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. with (NA-FI) or without 2 furosemide. The difference of both assays represents the NA-FS activity. Five independent experiments were carried out. ANOVA was used as statistical analysis (p<0.05). After 240 min NA-FI activity increased in 35‰S (t0=20.8±5.9 and t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. activities were determined at 15, 30, 60, 120 and 240min in homogenates (0.25MSucrose/0.5mMEGTA-Tris pH7.4) by measuring (13mM) ATP hydrolysis in (mM): 20imidazole (pH7.4) / 100NaCl /0.5EGTA. NKA: was determined as the difference between total (30KCl) and basal activity (without KCl/1 ouabain). NA activity was determined in the presence of 1ouabain/1NaN3, with (NA-FI) or without 2 furosemide. The difference of both assays represents the NA-FS activity. Five independent experiments were carried out. ANOVA was used as statistical analysis (p<0.05). After 240 min NA-FI activity increased in 35‰S (t0=20.8±5.9 and t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. with (NA-FI) or without 2 furosemide. The difference of both assays represents the NA-FS activity. Five independent experiments were carried out. ANOVA was used as statistical analysis (p<0.05). After 240 min NA-FI activity increased in 35‰S (t0=20.8±5.9 and t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. to furosemide (FI) activities in chela muscle. Male adult crabs acclimated at 10‰ and 35‰ salinity (S) were totally submerged for 24h (t0) and then exposed to air up to 240min. ATPase activities were determined at 15, 30, 60, 120 and 240min in homogenates (0.25MSucrose/0.5mMEGTA-Tris pH7.4) by measuring (13mM) ATP hydrolysis in (mM): 20imidazole (pH7.4) / 100NaCl /0.5EGTA. NKA: was determined as the difference between total (30KCl) and basal activity (without KCl/1 ouabain). NA activity was determined in the presence of 1ouabain/1NaN3, with (NA-FI) or without 2 furosemide. The difference of both assays represents the NA-FS activity. Five independent experiments were carried out. ANOVA was used as statistical analysis (p<0.05). After 240 min NA-FI activity increased in 35‰S (t0=20.8±5.9 and t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. with (NA-FI) or without 2 furosemide. The difference of both assays represents the NA-FS activity. Five independent experiments were carried out. ANOVA was used as statistical analysis (p<0.05). After 240 min NA-FI activity increased in 35‰S (t0=20.8±5.9 and t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. activities were determined at 15, 30, 60, 120 and 240min in homogenates (0.25MSucrose/0.5mMEGTA-Tris pH7.4) by measuring (13mM) ATP hydrolysis in (mM): 20imidazole (pH7.4) / 100NaCl /0.5EGTA. NKA: was determined as the difference between total (30KCl) and basal activity (without KCl/1 ouabain). NA activity was determined in the presence of 1ouabain/1NaN3, with (NA-FI) or without 2 furosemide. The difference of both assays represents the NA-FS activity. Five independent experiments were carried out. ANOVA was used as statistical analysis (p<0.05). After 240 min NA-FI activity increased in 35‰S (t0=20.8±5.9 and t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. with (NA-FI) or without 2 furosemide. The difference of both assays represents the NA-FS activity. Five independent experiments were carried out. ANOVA was used as statistical analysis (p<0.05). After 240 min NA-FI activity increased in 35‰S (t0=20.8±5.9 and t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. ATPase activities with differential response to salinity. The aim of this work was to determine the response to emersion of Na+K+ATPase (NKA) and Na+ATPase (NA) sensitive (FS) and insensitive to furosemide (FI) activities in chela muscle. Male adult crabs acclimated at 10‰ and 35‰ salinity (S) were totally submerged for 24h (t0) and then exposed to air up to 240min. ATPase activities were determined at 15, 30, 60, 120 and 240min in homogenates (0.25MSucrose/0.5mMEGTA-Tris pH7.4) by measuring (13mM) ATP hydrolysis in (mM): 20imidazole (pH7.4) / 100NaCl /0.5EGTA. NKA: was determined as the difference between total (30KCl) and basal activity (without KCl/1 ouabain). NA activity was determined in the presence of 1ouabain/1NaN3, with (NA-FI) or without 2 furosemide. The difference of both assays represents the NA-FS activity. Five independent experiments were carried out. ANOVA was used as statistical analysis (p<0.05). After 240 min NA-FI activity increased in 35‰S (t0=20.8±5.9 and t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. with (NA-FI) or without 2 furosemide. The difference of both assays represents the NA-FS activity. Five independent experiments were carried out. ANOVA was used as statistical analysis (p<0.05). After 240 min NA-FI activity increased in 35‰S (t0=20.8±5.9 and t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. granulatus and suggest a role of NA-FI activity in biochemical adjustments upon emersion. adjustments upon emersion. activities were determined at 15, 30, 60, 120 and 240min in homogenates (0.25MSucrose/0.5mMEGTA-Tris pH7.4) by measuring (13mM) ATP hydrolysis in (mM): 20imidazole (pH7.4) / 100NaCl /0.5EGTA. NKA: was determined as the difference between total (30KCl) and basal activity (without KCl/1 ouabain). NA activity was determined in the presence of 1ouabain/1NaN3, with (NA-FI) or without 2 furosemide. The difference of both assays represents the NA-FS activity. Five independent experiments were carried out. ANOVA was used as statistical analysis (p<0.05). After 240 min NA-FI activity increased in 35‰S (t0=20.8±5.9 and t240=32.9±5.9 nmolPixmin-1xmgprot-1). No changes occurred in NAFI at 10‰S either NKA and NA-FS. Our results show a differential response of ATPase activities to emersion in muscle of C. granulatus