INVESTIGADORES
MARIN Maia Solange
congresos y reuniones científicas
Título:
EFFECTS OF TLR7/8 STIMULATION ON BOVINE HERPESVIRUS TYPES 1 AND 5 REPLICATION
Autor/es:
MARIN MS; PÉREZ SE; LEUNDA MR; FAVERÍN C; ODEÓN AC
Lugar:
Mar del Plata
Reunión:
Encuentro; VII Encuentro Biólogos en Red; 2012
Institución organizadora:
AJIF
Resumen:
Studies on the effect of Toll like receptors (TLR) activation on Bovine Herpesvirus(BoHV) replication have not been reported. To test the effect of immunological effectorsexpressed after stimulation of TLR on BoHV-1 and 5 replication, PBMC from serum-negativecalves were stimulated with Imiquimod, a TLR7/8 agonist, for 6 or 24 h. Supernatants fromstimulated PBMC were harvested and added to MDBK cells previously infected with BoHV-1 orBoHV-5 strains for 0, 4, 6 or 24 h (M.O.I 0.1). Supernatants were harvested at 24 h and virustitres were determined by the end-point titration method. Three replicas of each experimentwere performed. As negative control, supernatants from non-stimulated PBMC were added toinfected MDBK cells. Supernatants obtained after PBMC stimulation demonstrated to haveantiviral activity. Stimulation with Imiquimod for 6 and 24 h significantly decreased (p
