INVESTIGADORES
MARIN Maia Solange
congresos y reuniones científicas
Título:
TOLL LIKE RECEPTOR 3 EXPRESSION PATTERNS IN THE CENTRAL NERVOUS SYSTEM OF BOVINE HERPESVIRUS 5- INFECTED CALVES
Autor/es:
MARIN MS; PÉREZ SE; QUINTANA S; LEUNDA MR; PEREYRA SE; MOORE DP; ODEÓN AC
Lugar:
Lima
Reunión:
Congreso; X Congreso de la Asociación Latinoamericana de Inmunología ALAI 2012; 2012
Institución organizadora:
Asociación Latinoamericana de Inmunología ALAI
Resumen:
Bovine herpesvirus 5 (BoHV-5) is the causal agent of necrotizing non-suppurative meningoencephalitis in cattle. In humans, a
deficiency of Toll like receptor 3
(TLR3) in the central nervous system (CNS) has
been associated with the development ofherpes simplex virusencephalitis. The aim of this study was to determine the relative expression
of TLR3 in the CNS of calves infected with BoHV-5 during acute infection, latency
and viral reactivation. Twelve calves were inoculated withBoHV-5 at doses of high titer and they were slaughtered during
the peak of acute infection.
Thirteen calves were inoculated with a low titer doseto induce establishment of latency.Two of them were
sacrificed 2 months post-inoculation
while the remaining calves were treated
with dexamethasone prior to slaughter to stimulate reactivation. Uninfected calves were used as control. Total RNA from several areas of the frontal
cortex and trigeminal ganglia of calves was isolated by using Trizol, digested with DNase
I and cDNA was synthesized from 1 µg of RNA. Real time RT-PCR
was applied for quantification
of TLR mRNA levels. The expression of TRL3 was measured and statistical
analysis was performed by using the Relative Expression Software Tool. TLR3 expression was detected in uninfected calves. Up-regulation of TLR3
during acute infection and viral reactivation was observed in some areas of
frontal cortex. Statistically significant differences were not detected in TLR3
expression levels in CNS from latently infected calves and in trigeminal
ganglia at any stage of infection in neither case. This work is the first
report associating TLR3 expression levels to
herpesviral infection in cattle. In-depth
descriptions of the protective mechanisms of TLR3 will greatly contribute to
its clinical application. Our findings also contribute to the understanding and
knowledge of BoHV pathogenesis.