Hepatocyte growth factor (HGF) induces vascular leakage in the retina

LIMA E SILVA R; CAMPOCHIARO PA; LORENC VE; FORTMANN SD

Hawaii

Congreso; ARVO (Association for Research in Vision and Opthalmology) 2018 Annual Meeting; 2018

Purpose: To investigate the effects of hepatocyte growth factor (HGF) on retinal vascular leakage and compare withleakage induced by vascular endothelial growth factor (VEGF)Methods: Adult C57BL/6 mice were given an intravitreous injection of 0.5 μg (6.25 μM) HGF or 0.13 μg (6.25 μM)VEGF, or 0.5 μg HGF + 0.13 μg VEGF in one eye and PBS in the fellow eye. At 1 and 3 days after injection (n = 8 foreach time point), the amount of albumin in the vitreous and retina was measured by ELISA. At 1 and 3 days afterinjection of 0.5 μg of HGF or PBS (n =5 for each time point), the level of mRNA for murine VEGF was measured byquantitative RT-PCRResults: Compared with fellow eyes injected with PBS, those injected with HGF had significantly more albumin in thevitreous (0.1 vs 0.06 μg/μl, p=0.01) and in the retina (0.009 vs 0.003 μg/μg, p=0.045) 1 day after injection. Thisdifference was maintained, 3 days after injection (0.09 vs 0.07 μg/μl in the vitreous, p=0.02; and 0.007 vs 0.004 μg/μgin the retina, p=0.012). Interesting the injection of both growth factors together increased the albumin content in thevitreous 1 day after injection, compare with each growth factor by itself (0.64 vs 0.28 μg/μl compared to VEGF,p