INVESTIGADORES
ULLOA DE LA SERNA Rita Maria
congresos y reuniones científicas
Título:
DIFFERENTIALLY EXPRESSED GENES IN POTATO
Autor/es:
GRANDELLIS C; GIAMMARIA V; BACHMANN SD; CERRUDO I; ULLOA RM
Lugar:
Carlos Paz, Córdoba
Reunión:
Congreso; XLIV Reunión Anual de la Sociedad Argentina de Investigación en; 2008
Institución organizadora:
SAIB
Resumen:
Tuber sprouting initiates after a period of dormancy under favorable
conditions. In order to analyze genes expressed upon sprouting in
different light conditions, tubers were grown for 10 days under
continuous light (10L) or for 10 or 35 days under complete darkness
(10D, 35D). Total RNA was obtained from sprouts, labeled with
Cy3 or Cy5 and hybridized to potato microarrays produced by
TIGR. A group of genes related to photosynthesis, carbon
metabolism and light perception was enhanced (>5 fold induction)
in 10L (light induced genes) while in 35 days etiolated sprouts
different proteinase inhibitors (PIs): Kunitz-type PIs, protease
inhibitor seed storage lipid transfer protein (LTP),
Metallocarboxypeptidase inhibitor IIa (MCPI2) were drastically
induced (13-fold). These results were confirmed by RT-PCR. PIs
play a potent defensive role against predators and pathogens and
interfere with physiological processes such as germination and
maturation. PIs may regulate cell proteolysis by inhibition of
endogenous proteases and thus control protein turnover and
metabolism. Diverse functions have been proposed for PIs, ranging
from regulators of endogenous proteinases to storage proteins. In
chick pea, PIs could be involved in xylem vessel formation and a
cell wall PI in the elongation process. We suggest that in potato, PIs
might be involved in regulating the elongation of etiolated
sprouts.