AQP3 IS REQUIRED FOR EXTRAVILLOUS TROPHOBLAST MIGRATION DURING PLACENTATION

MARTÍNEZ N.; RECA A.; REPPETTI J.; SEYAHIAN A.; SZPILBARG N.; DAMIANO A. E.

Buenos Aires

Congreso; Reunión conjunta de Sociedades de Biociencias; 2017

Development of the human placenta is critical for a successfulpregnancy. It requires that the extravillous cytotrophoblast cells(EVTs) migrate, invade and transform maternal spiral arteries. Itwas recently reported that aquaporin 3 (AQP3) is expressed in trophoblastcells during early gestation and its expression decreaseddramatically in preeclamptic placentas. Although, AQP3 is involvedin the migration of different cell types, its role in human placenta isstill unknown.Objective: To evaluate the role of AQP3 in the migration and theinvasion of EVT.Methods: Trophoblast cells (Swan 71 cell line) were cultured incomplete DMEM-F12. AQP3 expression was examined by Westernblot, RT-PCR and immunofluorescence. Cells were also treated with100 μM CuSO4 (AQP3 specific blocker) or SiRNA-AQP3 to inhibitAQP3. Migration was assessed by wound healing assay. Transwellinvasion assay was also performed. Metalloproteinase activitieswere evaluated by gelatin gel zymography.Results: AQP3 expression was found in Swan-71. Wound healingassay: the closure of the gap expressed as Control vs CuSO4 (n=5)at each time point was: 8 h (20.77 ±1.23 vs 14.44± 1.21, p