LACTIC ACID BATERIA: DESIGN AND EVALUATION OF A PRODUCT FOR THE PREVENTION OF BOVINE MASTITIS

BERARDO N.; SANTAMARINA S.; NADER-MACIAS, MEF.; GIRAUDO J.; BOGNI C.; PELLEGRINO M.

Capital Federal

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS; 2017

Sociedades de Biociencias

Abstract: In order to develop a natural product for the prevention of bovine mastitis, the effect of excipients and phytocompounds on the viability of two potential probiotic Lactic Acid Bacteria (LAB) and after inoculation in mammary glands was evaluated. The viability of LAB against several compounds (Asian Centella, Echinacea, Aloe vera, Vitamins C and D, Fructose and Sepigel) was evaluated by minimal inhibitory concentration and death curves. No inhibition was observed for phytocompounds at any of the tested concentrations and no significant differences (p0.05) the growth of both LAB at higher concentrations (25, 50 and 100mg/ml). Fructose delays the growth of LAB at 300mg/ml and cause aninhibitory effect at 600mg/ml. The effect of selected compounds and concentrations were determined in an experimental inoculation trial in cows by Somatic Cell Count (SCC) and clinical signs and symptoms determinations. For this, healthy cows were inoculated intramammarily with Echinacea (6.25mg/ml), Fructose (10mg/ml), Sepigel (1%), LAB (3x106 cfu/ml), Diluent (Echinacea+fructose+Sepigel) or Product (LAB + diluent). None of the inoculated quarters showed adverse reactions and no anomalies were detected either at the local or systemic level. The quarters inoculated with Equinacea, LAB and product, showed a statistically significant increase (p0.05) respect to the control. The results allow concluding that it was possible to obtain a complete probiotic formulation for the prevention of bovine mastitis and thus helps to reduce the use of antibiotics as a prophylactic measure.